R Y Tsien

1. Wavelength mutations and posttranslational autoxidation of green fluorescent protein.

   Heim R, Prasher Dc, Tsien Ry

   (1994). Proceedings of the National Academy of Sciences, 91(26) , 12501-12504. doi: 10.1073/pnas.91.26.12501. Article

   Primary Proteins:

   1. CFP
   2. H9
   3. P11
   4. P4
   5. P9

   Additional Proteins:

   1. ECFP

2. Improved green fluorescence

   Heim R, Cubitt Ab, Tsien Ry

   (1995). Nature, 373(6516) , 663-664. doi: 10.1038/373663b0. Article   Pubmed

   Primary Proteins:

   1. GFP (S65T)

   Additional Proteins:

   1. avGFP

3. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

   Heim R, Tsien Ry

   (1996). Current Biology, 6(2) , 178-182. doi: 10.1016/s0960-9822(02)00450-5. Article

   Primary Proteins:

   1. BFP
   2. ECFP
   3. P4-1
   4. W2
   5. W7

   Additional Proteins:

   1. GFP (S65T)

4. Crystal Structure of the Aequorea victoria Green Fluorescent Protein

   Orm  M, Cubitt Ab, Kallio K, Gross La, Tsien Ry, Remington Sj

   (1996). Science, 273(5280) , 1392-1395. doi: 10.1126/science.273.5280.1392. Article

   Primary Proteins:

   1. 10B
   2. 11
   3. 5B
   4. 6C
   5. EYFP

   Additional Proteins:

   1. avGFP

5. Crystal Structure and Photodynamic Behavior of the Blue Emission Variant Y66H/Y145F of Green Fluorescent Protein†

   Wachter Rm, King Ba, Heim R, Kallio K, Tsien Ry, Boxer Sg, Remington Sj

   (1997). Biochemistry, 36(32) , 9759-9765. doi: 10.1021/bi970563w. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. P4

6. THE GREEN FLUORESCENT PROTEIN

   Tsien Ry

   (1998). Annual Review of Biochemistry, 67(1) , 509-544. doi: 10.1146/annurev.biochem.67.1.509. Article   Pubmed

   Primary Proteins:

   1. W1C

   Additional Proteins:

   1. avGFP
   2. EBFP
   3. ECFP
   4. EGFP
   5. mEGFP
   6. mEmerald
   7. P4
   8. Topaz

7. Dynamic and quantitative Ca2+ measurements using improved cameleons

   Miyawaki A, Griesbeck O, Heim R, Tsien Ry

   (1999). Proceedings of the National Academy of Sciences, 96(5) , 2135-2140. doi: 10.1073/pnas.96.5.2135. Article

   Primary Proteins:

   1. EYFP-Q69K

8. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral

   Baird Gs, Zacharias Da, Tsien Ry

   (2000). Proceedings of the National Academy of Sciences, 97(22) , 11984-11989. doi: 10.1073/pnas.97.22.11984. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. DsRed

9. The structure of the chromophore within DsRed, a red fluorescent protein from coral

   Gross La, Baird Gs, Hoffman Rc, Baldridge Kk, Tsien Ry

   (2000). Proceedings of the National Academy of Sciences, 97(22) , 11990-11995. doi: 10.1073/pnas.97.22.11990. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. DsRed

10. Reducing the Environmental Sensitivity of Yellow Fluorescent Protein

    Griesbeck O, Baird Gs, Campbell Re, Zacharias Da, Tsien Ry

    (2001). Journal of Biological Chemistry, 276(31) , 29188-29194. doi: 10.1074/jbc.m102815200. Article   Pubmed

    Primary Proteins:

    1. Citrine
    2. cpCitrine

    Additional Proteins:

    1. mCitrine

11. A monomeric red fluorescent protein

    Campbell Re, Tour O, Palmer Ae, Steinbach Pa, Baird Gs, Zacharias Da, Tsien Ry

    (2002). Proceedings of the National Academy of Sciences, 99(12) , 7877-7882. doi: 10.1073/pnas.082243699. Article   Pubmed

    Primary Proteins:

    1. dimer1
    2. dimer2
    3. mRFP1
    4. tdimer2(12)

12. Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators

    Hanson Gt, Aggeler R, Oglesbee D, Cannon M, Capaldi Ra, Tsien Ry, Remington Sj

    (2004). Journal of Biological Chemistry, 279(13) , 13044-13053. doi: 10.1074/jbc.m312846200. Article   Pubmed

    Primary Proteins:

    1. roGFP1
    2. roGFP1-R1
    3. roGFP1-R8
    4. roGFP2

13. Evolution of new nonantibody proteins via iterative somatic hypermutation

    Wang L, Jackson Wc, Steinbach Pa, Tsien Ry

    (2004). Proceedings of the National Academy of Sciences, 101(48) , 16745-16749. doi: 10.1073/pnas.0407752101. Article   Pubmed

    Primary Proteins:

    1. mPlum
    2. mRaspberry

    Additional Proteins:

    1. mRFP1.2

14. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

    Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry

    (2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037. Article   Pubmed

    Primary Proteins:

    1. dTomato
    2. mBanana
    3. mCherry
    4. mHoneydew
    5. mOFP.T.12
    6. mOFP.T.8
    7. mOrange
    8. mRFP1.1
    9. mRFP1.3
    10. mRFP1.4
    11. mRFP1.5
    12. mStrawberry
    13. mTangerine
    14. tdTomato

    Additional Proteins:

    1. dimer2
    2. DsRed
    3. DsRed-Express
    4. mRFP1

15. A guide to choosing fluorescent proteins

    Shaner Nc, Steinbach Pa, Tsien Ry

    (2005). Nature Methods, 2(12) , 905-909. doi: 10.1038/nmeth819. Article   Pubmed

    Primary Proteins:

16. Novel Chromophores and Buried Charges Control Color in mFruits†,‡

    Shu X, Shaner Nc, Yarbrough Ca, Tsien Ry, Remington Sj

    (2006). Biochemistry, 45(32) , 9639-9647. doi: 10.1021/bi060773l. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. mCherry
    2. mOrange

17. Evolving proteins in mammalian cells using somatic hypermutation

    Wang L, Tsien Ry

    (2006). Nature Protocols, 1(3) , 1346-1350. doi: 10.1038/nprot.2006.243. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. mRFP1.2

18. Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins†

    Ai H-, Shaner Nc, Cheng Z, Tsien Ry, Campbell Re

    (2007). Biochemistry, 46(20) , 5904-5910. doi: 10.1021/bi700199g. Article   Pubmed

    Primary Proteins:

    1. EBFP1.2
    2. EBFP1.5
    3. EBFP2
    4. mBlueberry1
    5. mBlueberry2
    6. mKalama1

19. Improving the photostability of bright monomeric orange and red fluorescent proteins

    Shaner Nc, Lin Mz, Mckeown Mr, Steinbach Pa, Hazelwood Kl, Davidson Mw, Tsien Ry

    (2008). Nature Methods, 5(6) , 545-551. doi: 10.1038/nmeth.1209. Article   Pubmed

    Primary Proteins:

    1. mApple
    2. mOrange2
    3. TagRFP-T

    Additional Proteins:

    1. mEGFP

20. Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics

    Lin Jy, Lin Mz, Steinbach P, Tsien Ry

    (2009). Biophysical Journal, 96(5) , 1803-1814. doi: 10.1016/j.bpj.2008.11.034. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. Channelrhodopsin2

21. Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

    Shu X, Royant A, Lin Mz, Aguilera Ta, Lev-Ram V, Steinbach Pa, Tsien Ry

    (2009). Science, 324(5928) , 804-807. doi: 10.1126/science.1168683. Article   Pubmed

    Primary Proteins:

    1. DrCBD
    2. iFP1.4

22. Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals

    Lin Mz, Mckeown Mr, Ng H-L, Aguilera Ta, Shaner Nc, Campbell Re, Adams Sr, Gross La, Ma W, Alber T, Tsien Ry

    (2009). Chemistry & Biology, 16(11) , 1169-1179. doi: 10.1016/j.chembiol.2009.10.009. Article   Pubmed

    Primary Proteins:

    1. mGrape1
    2. mGrape2
    3. mGrape3
    4. mKate M41G S158C
    5. mKate S158C
    6. mNeptune
    7. Neptune

    Additional Proteins:

    1. mKate S158A

23. A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

    Shu X, Lev-Ram V, Deerinck Tj, Qi Y, Ramko Eb, Davidson Mw, Jin Y, Ellisman Mh, Tsien Ry

    (2011). PLoS Biology, 9(4) , e1001041. doi: 10.1371/journal.pbio.1001041. Article   Pubmed

    Primary Proteins:

    1. miniSOG

24. Improving FRET dynamic range with bright green and red fluorescent proteins

    Lam Aj, St-Pierre F, Gong Y, Marshall Jd, Cranfill Pj, Baird Ma, Mckeown Mr, Wiedenmann J, Davidson Mw, Schnitzer Mj, Tsien Ry, Lin Mz

    (2012). Nature Methods, 9(10) , 1005-1012. doi: 10.1038/nmeth.2171. Article   Pubmed

    Primary Proteins:

    1. Clover
    2. mRuby2

    Additional Proteins:

    1. eqFP611

25. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein

    Rodriguez Ea, Tran Gn, Gross La, Crisp Jl, Shu X, Lin Jy, Tsien Ry

    (2016). Nature Methods, 13(9) , 763-769. doi: 10.1038/nmeth.3935. Article   Pubmed

    Primary Proteins:

    1. R3-2+PCB
    2. smURFP
    3. TDsmURFP
    4. TeAPCα