FPbase FRET Calculator

QY = Quantum Yield, EC = Extinction Coefficient, J(λ) = Overlap Integral, R0 = Förster Radius, 𝓃 = refractive index, κ2 = orientation factor
Wu & Brand (1994). Resonance Energy Transfer: Methods and Applications. Analytical Biochem. 218

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Förster Radii for all monomeric FP pairs in the database
Show Donor Acceptor λmaxD Δλem QYD εA QYA J(λ) R0 (Å) R0×QYA
Show Donor Acceptor λmaxD Δλem QYD εA QYA J(λ) R0 R0×QYA