|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|72.9 ± 5.5 (10000 cells)||-||HeLa||Cranfill et al. (2016)|
|72.9 ± 5.5 (10000 cells)||-||HeLa||Shaner et al. (2013)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|50.0||Shaner et al. (2013)|
Clover was derived from avGFP with the following mutations: M1_S2insV/S30R/Y39N/S65G/Q69A/F99S/N105T/Y145F/M153T/V163A/I171V/T203H
With a completely deprotonated chromophore at neutral pH (pKa = 6.1), Clover should be incapable of photoconversion despite the presence of His203, which enhances photoconversion in PA-GFP. Indeed, we observed no photoactivation of Clover in illumination conditions that produced more than threefold photoactivation of PA-GFP.