a.k.a. equaRFP
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Tetramer | Entacmaea quadricolor | 26.1 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 559 | 611 | 78,000 | 0.45 | 35.1 | 3.84 | 270.0 | 2.5 |
No photostability measurements available ... add one!
The protein has a reduced tendency to oligomerize, as shown by its monomeric appearance in SDS/PAGE analysis and single-molecule experiments. However, it forms tetramers at higher concentrations in the absence of detergent.
Wiedenmann et al. (2002)
Together, eqFP578 and eqFP611 represent a separate group of red fluorescent proteins, which are characterized by high fluorescence brightness, complete chromophore maturation and, notably, a reduced tendency to oligomerize. These features make E. quadricolor fluorescent proteins an attractive starting point for the generation of bright monomeric red fluorescent proteins.
Merzlyak et al. (2007)
The precursor to mRuby, eqFP611 dimer, readily undergoes reversible photobleaching due to isomerization from a bright trans form to a dim cis form, and mutations near the chromophore affect isomerization
Lam et al. (2012)
(2002). Proceedings of the National Academy of Sciences, 99(18) , 11646-11651. doi: 10.1073/pnas.182157199. Article Pubmed
(2012). Nature Methods, 9(10) , 1005-1012. doi: 10.1038/nmeth.2171. Article Pubmed
(2008). Marine Biotechnology, 10(3) , 328-342. doi: 10.1007/s10126-007-9072-7. Article Pubmed
(2008). Chemistry & Biology, 15(3) , 224-233. doi: 10.1016/j.chembiol.2008.02.008. Article Pubmed
(2007). Nature Methods, 4(7) , 555-557. doi: 10.1038/nmeth1062. Article Pubmed
(2005). Journal of Biomedical Optics, 10(1) , 014003. doi: 10.1117/1.1854680. Article Pubmed
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