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Comparison List

dTomato

dTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a somewhat slowly-maturing dimer with low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Discosoma sp. 27.0 kDa -

FPbase ID: G1DQY

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
554 581 69,000 0.69 47.61 4.7 60.0  
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dTomato OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
31.0 (258 cells) 5.7 ± 0.7 (110 cells) U-2 OS Bindels et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
64.0   Shaner et al. (2004)
A caution on interpretation of photostability measurements
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dTomato Sequence

BLAST

dTomato was derived from dimer2 with the following mutations: A2_D6delinsSKGEE/V22M/Q66M/V104L/F124M/*224YextGMDELYK
amino acid numbers relative to DsRed. show relative to dimer2

MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLYGMDELYK
GenBank: AAV52168
IPG: 3838230

Excerpts

No excerpts have been added for dTomato
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry

(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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