|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|85.0||Kremers et al. (2006)|
ECFP was derived from W7 with the following mutations: M1_S2insV/F64L/S65T/K212N/H231L
Note: The ECFP line is sometimes incorrectly reported as having the mutation N149I (instead of N146I) with respect to wild-type avGFP (e.g. see Supp Table 2 of Shaner et al 2005 ... since corrected in the online media). The correct mutation is N146I, which first appeared in the W7 mutant (Heim & Tsien 1996), from which most CFPs are evolved.
No excerpts have been added for ECFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).
(1996). Current Biology, 6(2) , 178-182. doi: 10.1016/s0960-9822(02)00450-5. Article
(1998). Methods in Cell Biology, , 19-30. doi: 10.1016/s0091-679x(08)61946-9. Article
(1994). Proceedings of the National Academy of Sciences, 91(26) , 12501-12504. doi: 10.1073/pnas.91.26.12501. Article