A Miyawaki

1. Dynamic and quantitative Ca2+ measurements using improved cameleons

   Miyawaki A, Griesbeck O, Heim R, Tsien Ry

   (1999). Proceedings of the National Academy of Sciences, 96(5) , 2135-2140. doi: 10.1073/pnas.96.5.2135. Article

   Primary Proteins:

   1. EYFP-Q69K

2. Circularly permuted green fluorescent proteins engineered to sense Ca2+

   Nagai T, Sawano A, Park Es, Miyawaki A

   (2001). Proceedings of the National Academy of Sciences, 98(6) , 3197-3202. doi: 10.1073/pnas.051636098. Article   Pubmed

   Primary Proteins:

   1. cpEYFP(V68L/Q69K)

3. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

   Nagai T, Ibata K, Park Es, Kubota M, Mikoshiba K, Miyawaki A

   (2002). Nature Biotechnology, 20(1) , 87-90. doi: 10.1038/nbt0102-87. Article   Pubmed

   Primary Proteins:

   1. EYFP-F46L
   2. SEYFP
   3. Venus

4. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein

   Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A

   (2002). Proceedings of the National Academy of Sciences, 99(20) , 12651-12656. doi: 10.1073/pnas.202320599. Article   Pubmed

   Primary Proteins:

   1. Kaede

5. Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity

   Rekas A, Alattia J-R, Nagai T, Miyawaki A, Ikura M

   (2002). Journal of Biological Chemistry, 277(52) , 50573-50578. doi: 10.1074/jbc.m209524200. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Venus

6. A Green-emitting Fluorescent Protein fromGalaxeidaeCoral and Its Monomeric Version for Use in Fluorescent Labeling

   Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A

   (2003). Journal of Biological Chemistry, 278(36) , 34167-34171. doi: 10.1074/jbc.m304063200. Article   Pubmed

   Primary Proteins:

   1. AzamiGreen
   2. mAzamiGreen

7. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter

   Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A

   (2005). EMBO reports, 6(3) , 233-238. doi: 10.1038/sj.embor.7400361. Article   Pubmed

   Primary Proteins:

   1. KikG
   2. KikGR1

8. From The Cover: Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa

   Habuchi S, Ando R, Dedecker P, Verheijen W, Mizuno H, Miyawaki A, Hofkens J

   (2005). Proceedings of the National Academy of Sciences, 102(27) , 9511-9516. doi: 10.1073/pnas.0500489102. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Dronpa

9. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

   Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A

   (2006). Nature Biotechnology, 24(5) , 577-581. doi: 10.1038/nbt1207. Article   Pubmed

   Primary Proteins:

   1. dKeima
   2. dKeima570
   3. mKeima
   4. Montipora sp. #20
   5. Montipora sp. #20-9115
   6. tKeima

10. Concatenation of Cyan and Yellow Fluorescent Proteins for Efficient Resonance Energy Transfer†

    Shimozono S, Hosoi H, Mizuno H, Fukano T, Tahara T, Miyawaki A

    (2006). Biochemistry, 45(20) , 6267-6271. doi: 10.1021/bi060093i. Article   Pubmed

    Primary Proteins:

    1. Cy11.5

11. Highlighted Generation of Fluorescence Signals Using Simultaneous Two-Color Irradiation on Dronpa Mutants

    Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A

    (2007). Biophysical Journal, 92(12) , L97-L99. doi: 10.1529/biophysj.107.105882. Article   Pubmed

    Primary Proteins:

    1. Dronpa-2
    2. Dronpa-3

12. Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

    Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A

    (2008). Cell, 132(3) , 487-498. doi: 10.1016/j.cell.2007.12.033. Article   Pubmed

    Primary Proteins:

    1. mKO2

13. Sequential Binding of Cytosolic Phox Complex to Phagosomes through Regulated Adaptor Proteins: Evaluation Using the Novel Monomeric Kusabira-Green System and Live Imaging of Phagocytosis

    Ueyama T, Kusakabe T, Karasawa S, Kawasaki T, Shimizu A, Son J, Leto Tl, Miyawaki A, Saito N

    (2008). The Journal of Immunology, 181(1) , 629-640. doi: 10.4049/jimmunol.181.1.629. Article

    Primary Proteins:

    1. mKG

14. Improving membrane voltage measurements using FRET with new fluorescent proteins

    Tsutsui H, Karasawa S, Okamura Y, Miyawaki A

    (2008). Nature Methods, 5(8) , 683-685. doi: 10.1038/nmeth.1235. Article   Pubmed

    Primary Proteins:

    1. mKOκ
    2. mUkG

15. Transgenic-Cloned Pigs Systemically Expressing Red Fluorescent Protein, Kusabira-Orange

    Matsunari H, Onodera M, Tada N, Mochizuki H, Karasawa S, Haruyama E, Nakayama N, Saito H, Ueno S, Kurome M, Miyawaki A, Nagashima H

    (2008). Cloning and Stem Cells, 10(3) , 313-324. doi: 10.1089/clo.2008.0024. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. KO

16. mKikGR, a Monomeric Photoswitchable Fluorescent Protein

    Habuchi S, Tsutsui H, Kochaniak Ab, Miyawaki A, Van Oijen Am

    (2008). PLoS ONE, 3(12) , e3944. doi: 10.1371/journal.pone.0003944. Article   Pubmed

    Primary Proteins:

    1. mKikGR

17. Crystal Structure of a New Cyan Fluorescent Protein and Its Hue-Shifted Variants,

    Kikuchi A, Fukumura E, Karasawa S, Shiro Y, Miyawaki A

    (2009). Biochemistry, 48(23) , 5276-5283. doi: 10.1021/bi801658p. Article   Pubmed

    Primary Proteins:

    1. KCY
    2. KCY-G4219
    3. KCY-G4219-38L
    4. KCY-R1
    5. KCY-R1-158A
    6. KCY-R1-38H
    7. KCY-R1-38L

18. Higher resolution in localization microscopy by slower switching of a photochromic protein

    Mizuno H, Dedecker P, Ando R, Fukano T, Hofkens J, Miyawaki A

    (2010). Photochemical & Photobiological Sciences, 9(2) , 239. doi: 10.1039/b9pp00124g. Article   Pubmed

    Primary Proteins:

    1. PDM1-4

19. Age-dependent Preferential Dense-Core Vesicle Exocytosis in Neuroendocrine Cells Revealed by Newly Developed Monomeric Fluorescent Timer Protein

    Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A

    (2010). Molecular Biology of the Cell, 21(1) , 87-94. doi: 10.1091/mbc.e09-08-0722. Article   Pubmed

    Primary Proteins:

    1. mK-GO

    Additional Proteins:

    1. DsRed-Timer

20. Data storage based on photochromic and photoconvertible fluorescent proteins

    Adam V, Mizuno H, Grichine A, Hotta J-, Yamagata Y, Moeyaert B, Nienhaus Gu, Miyawaki A, Bourgeois D, Hofkens J

    (2010). Journal of Biotechnology, 149(4) , 289-298. doi: 10.1016/j.jbiotec.2010.04.001. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. IrisFP

21. Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications

    Adam V, Moeyaert B, David Cc, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J

    (2011). Chemistry & Biology, 18(10) , 1241-1251. doi: 10.1016/j.chembiol.2011.08.007. Article   Pubmed

    Primary Proteins:

    1. Dendra2-M159A
    2. mEosFP-F173S
    3. mEosFP-M159A
    4. NijiFP

    Additional Proteins:

    1. Dendra2
    2. mEosFP

22. A Bilirubin-Inducible Fluorescent Protein from Eel Muscle

    Kumagai A, Ando R, Miyatake H, Greimel P, Kobayashi T, Hirabayashi Y, Shimogori T, Miyawaki A

    (2013). Cell, 153(7) , 1602-1611. doi: 10.1016/j.cell.2013.05.038. Article   Pubmed

    Primary Proteins:

    1. UnaG

23. A Diffraction-Quality Protein Crystal Processed as an Autophagic Cargo

    Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A

    (2015). Molecular Cell, 58(1) , 186-193. doi: 10.1016/j.molcel.2015.02.007. Article   Pubmed

    Primary Proteins:

    1. Xpa

24. Photoconvertible Behavior of LSSmOrange Applicable for Single Emission Band Optical Highlighting

    De Keersmaecker H, Fron E, Rocha S, Kogure T, Miyawaki A, Hofkens J, Mizuno H

    (2016). Biophysical Journal, 111(5) , 1014-1025. doi: 10.1016/j.bpj.2016.07.033. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. LSSmOrange

25. Two Distinct Fluorescence States of the Ligand-Induced Green Fluorescent Protein UnaG

    Shitashima Y, Shimozawa T, Kumagai A, Miyawaki A, Asahi T

    (2017). Biophysical Journal, 113(12) , 2805-2814. doi: 10.1016/j.bpj.2017.10.022. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. UnaG

26. Coupling delay controls synchronized oscillation in the segmentation clock

    Yoshioka-Kobayashi K, Matsumiya M, Niino Y, Isomura A, Kori H, Miyawaki A, Kageyama R

    (2020). Nature, , . doi: 10.1038/s41586-019-1882-z. Article   Pubmed

    Primary Proteins:

    1. Achilles

27. Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

    Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A

    (2020). , , . doi: 10.1101/2020.03.30.015156. Article

    Primary Proteins:

    1. AzaleaB5
    2. h2-3

28. A highly photostable and bright green fluorescent protein

    Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A

    (2022). Nature Biotechnology, , . doi: 10.1038/s41587-022-01278-2. Article   Pubmed

    Primary Proteins:

    1. StayGold