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Comparison List

dimer2

dimer2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2002, derived from Discosoma sp.. It is reported to be a very slowly-maturing dimer with low acid sensitivity.

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Aggregation Organism Molecular Weight Cofactor
Dimer Discosoma sp. 25.9 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
552 579 69,000 0.69 47.61 4.9 120.0  

Photostability

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dimer2 Sequence

dimer2 was derived from dimer1 with the following mutations: V71A/C117T/F118L/V127T/S131P/A217S
amino acid numbers relative to DsRed. show relative to dimer1

MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFL
GenBank: AAM54542
IPG: 142581

Excerpts

The dimer2 variant possesses many desirable properties, such as a faster and more complete maturation than wild-type DsRed and a greater fluorescent brightness than the fast-maturing mutant DsRed.T1

Shaner et al. (2004)

Primary Reference

A monomeric red fluorescent protein

Campbell Re, Tour O, Palmer Ae, Steinbach Pa, Baird Gs, Zacharias Da, Tsien Ry

(2002). Proceedings of the National Academy of Sciences, 99(12) , 7877-7882. doi: 10.1073/pnas.082243699. Article   Pubmed

Additional References

External Resources

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