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Comparison List

dimer2

dimer2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2002, derived from Discosoma sp.. It has low acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Dimer Discosoma sp. 25.9 kDa -

FPbase ID: DFXUK

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
552 579 69,000 0.69 47.61 4.9 120.0  
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Photostability

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dimer2 Sequence

BLAST

dimer2 was derived from dimer1 with the following mutations: V71A/C117T/F118L/V127T/S131P/A217S
amino acid numbers relative to DsRed. show relative to dimer1

MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFL
GenBank: AAM54542
IPG: 142581

Excerpts

The dimer2 variant possesses many desirable properties, such as a faster and more complete maturation than wild-type DsRed and a greater fluorescent brightness than the fast-maturing mutant DsRed.T1

Shaner et al. (2004)

Primary Reference

A monomeric red fluorescent protein

Campbell Re, Tour O, Palmer Ae, Steinbach Pa, Baird Gs, Zacharias Da, Tsien Ry

(2002). Proceedings of the National Academy of Sciences, 99(12) , 7877-7882. doi: 10.1073/pnas.082243699. Article   Pubmed

Additional References

  1. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

    Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry

    (2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037. Article   Pubmed

External Resources

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