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Comparison List

iFP1.4

iFP1.4 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2009, derived from Deinococcus radiodurans. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Deinococcus radiodurans 34.8 kDa Biliverdin

FPbase ID: WSRVG

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
684 708 92,000 0.07 6.44 4.6 114.0  
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
50.0   Shu et al. (2009)
A caution on interpretation of photostability measurements
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iFP1.4 Sequence

BLAST

iFP1.4 was derived from DrCBD with the following mutations: S2A/M54V/G119A/T135I/V186M/L195M/H196Q/D207H/I208T/A288V/Y307E/L311K/L314G/V318R

MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADGHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLDWPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTVRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLRLTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEA

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for iFP1.4
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

Shu X, Royant A, Lin Mz, Aguilera Ta, Lev-Ram V, Steinbach Pa, Tsien Ry

(2009). Science, 324(5928) , 804-807. doi: 10.1126/science.1168683. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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