similar: EGFP
Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Aequorea victoria | 27.0 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
488 | 507 | 56,000 | 0.6 | 33.6 | 6.0 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
98.1 ± 1.6 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
98.1 ± 1.6 (10000 cells) | - | HeLa | Shaner et al. (2013) |
97.0 ± 2.1 (10000 cells) | - | HeLa | Paez-Segala et al. (2015) |
96.8 ± 0.5 (150 cells) | - | - | Bajar et al. (2016) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
46.0 | 240.0 (uW) | Laser | Point Scanning Confocal | Cranfill et al. (2016) | |||
239.0 | 60.0 (uW) | Laser | Point Scanning Confocal | Cranfill et al. (2016) |
mEGFP was derived from EGFP with the following mutations: A206K
amino acid numbers relative to avGFP. show relative to EGFP
We replaced hydrophobic residues at the crystallographic interface of the dimer with positively charged residues (A206K, L221K, or F223R). These mutants were likewise expressed and purified, and their self-association was measured by analytical ultracentrifugation... We call these novel monomeric GFP variants mCFP, mGFP, and mYFP. These mutations caused no significant alterations in the spectral properties.
Zacharias (2002)
(2002). Science, 296(5569) , 913-916. doi: 10.1126/science.1068539. Article Pubmed
(2018). Scientific Reports, 8(1) , 10634. doi: 10.1038/s41598-018-28858-0. Article Pubmed
(2016). Nature Methods, 13(7) , 557-562. doi: 10.1038/nmeth.3891. Article Pubmed
(2008). Nature Methods, 5(6) , 545-551. doi: 10.1038/nmeth.1209. Article Pubmed
(1998). Annual Review of Biochemistry, 67(1) , 509-544. doi: 10.1146/annurev.biochem.67.1.509. Article Pubmed
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