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Comparison List

EBFP

EBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 1998, derived from Aequorea victoria.
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Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.9 kDa -

FPbase ID: 1LZV9

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
380 440 31,500 0.2 6.3      
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Photostability

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EBFP Sequence

BLAST

EBFP was derived from BFP with the following mutations: M1_S2insV/F64L/S65T/H231L

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

Excerpts

No excerpts have been added for EBFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Improved Fluorescence and Dual Color Detection with Enhanced Blue and Green Variants of the Green Fluorescent Protein

Yang T-T, Sinai P, Green G, Kitts Pa, Chen Y-T, Lybarger L, Chervenak R, Patterson Gh, Piston Dw, Kain Sr

(1998). Journal of Biological Chemistry, 273(14) , 8212-8216. doi: 10.1074/jbc.273.14.8212. Article

Additional References

  1. THE GREEN FLUORESCENT PROTEIN

    Tsien Ry

    (1998). Annual Review of Biochemistry, 67(1) , 509-544. doi: 10.1146/annurev.biochem.67.1.509. Article   Pubmed

  2. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy

    Patterson Gh, Knobel Sm, Sharif Wd, Kain Sr, Piston Dw

    (1997). Biophysical Journal, 73(5) , 2782-2790. doi: 10.1016/s0006-3495(97)78307-3. Article

External Resources

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