Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Discosoma sp. | 27.0 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
568 | 592 | 75,000 | 0.49 | 36.75 | 6.5 | 30.0 | 2.9 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
95.3 ± 1.7 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
87.0 (103 cells) | 2.2 ± 0.2 (14 cells) | U-2 OS | Bindels et al. (2016) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
4.8 | Bindels et al. (2016) |
mApple was derived from mOrange with the following mutations: R17H/G40A/T66M/A71V/V73I/K92R/V104I/V105I/T106H/T108N/E117V/S147E/G159S/M163K/T174A/S175A/G196D/T202V
amino acid numbers relative to DsRed. show relative to mOrange
Six generations of directed evolution [of mOrange] with constant photostability selection yielded the variant mApple, which, though substantially brighter than mCherry, displayed complex photoswitching behavior. This behavior was more pronounced with continuous wide-field than with laser-scanning illumination and could be largely eliminated by excitation at alternate wavelengths or by intermittent illumination. However, given our later results using the brighter TagRFP as starting material, we chose not to pursue mApple any further.
Shaner et al. (2008)
(2008). Nature Methods, 5(6) , 545-551. doi: 10.1038/nmeth.1209. Article Pubmed
(2016). Nature Methods, 14(1) , 53-56. doi: 10.1038/nmeth.4074. Article Pubmed
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