|Monomer||Discosoma sp.||25.4 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|5.9||Shaner et al. (2004)|
mHoneydew was derived from mRFP1.1 with the following mutations: Y67W/E144A/S146T/S147T/T195I/I197G
Note: Amino acid 147 is inconsistently reported: Fig 2 in Shaner et al. (2004) and patent WO2006024041A2 (Fig 48A) show 147S. However, the GenBank AAV52171, patent WO2006024041A2 (Fig 44), SnapGene, and Addgene 91760 sequences all have 147T. Here we use 147T which is likely to be found in most plasmids.
No excerpts have been added for mHoneydew
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).
(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037. Article Pubmed
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