compare

Comparison List

dimer1

dimer1 is a basic (constitutively fluorescent) orange fluorescent protein published in 2002, derived from Discosoma sp..

No spectrum has been submitted ... add a spectrum!

Aggregation Organism Molecular Weight Cofactor
Dimer Discosoma sp. 25.9 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
551 579          

Photostability

no photostability measurements available ... add one!

dimer1 Sequence

dimer1 was derived from DsRed-Express with the following mutations: M1_A2insV/S117C/I125R/K163Q/S179T/S197T

MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIYKVKFRGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERAEGRHHLFL

Excerpts

[Dimer1 is an intermediate DsRed mutant in the development dimer2 and mRFP1]. Dimer1 was somewhat better than wild-type DsRed both in terms of brightness and rate of maturation but had a substantial green peak equivalent to that of DsRed.T1. Dimer1 was also somewhat blue-shifted with an excitation maximum at 551 nm and an emission maximum at 579 nm.

Campbell et al. (2002)

Primary Reference

A monomeric red fluorescent protein

Campbell Re, Tour O, Palmer Ae, Steinbach Pa, Baird Gs, Zacharias Da, Tsien Ry

(2002). Proceedings of the National Academy of Sciences, 99(12) , 7877-7882. doi: 10.1073/pnas.082243699. Article   Pubmed

Additional References

    No additional references yet...

External Resources

Something missing or incorrect? Submit a change Submit a change