compare

Comparison List

mOrange2

mOrange2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2008, derived from Discosoma sp.. It is reported to be a very slowly-maturing monomer with high acid sensitivity.
+
Aggregation Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.8 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
549 565 58,000 0.6 34.8 6.5 270.0 2.7

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
91.8 ± 1.4 (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
228.0 Dean et al. (2015)

mOrange2 Sequence

mOrange2 was derived from mOrange with the following mutations: Q64H/F99Y/E160K/G196D
amino acid numbers relative to DsRed. show relative to mOrange

MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGFQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
GenBank: ABC66096
IPG: 5194148

Excerpts

No excerpts have been added for mOrange2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. High-Speed Multiparameter Photophysical Analyses of Fluorophore Libraries

    Dean Km, Davis Lm, Lubbeck Jl, Manna P, Friis P, Palmer Ae, Jimenez R

    (2015). Analytical Chemistry, 87(10) , 5026-5030. doi: 10.1021/acs.analchem.5b00607. Article   Pubmed

External Resources

Something missing or incorrect? Submit a change Submit a change