a.k.a. enhanced GFP, GFPmut1
similar: mEGFP
Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Weak dimer | Aequorea victoria | 26.9 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
488 | 507 | 55,900 | 0.6 | 33.54 | 6.0 | 25.0 | 2.6 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
76.5 ± 6.9 (10000 cells) | - | HeLa | Hoi et al. (2013) |
- | 3.89 ± 0.25 (50 cells) | U-2 OS | Costantini et al. (2012) |
76.5 ± 6.9 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
76.5 ± 6.9 (10000 cells) | - | HeLa | Shaner et al. (2013) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
174.0 | Arc-lamp | Widefield | none | 23.0 | Shaner et al. (2005) | ||
50.1 | 1.5 (mW) | Laser | Point Scanning Confocal | Hela | Zhong et al. (2018) |
EGFP was derived from avGFP with the following mutations: M1_S2insV/F64L/S65T/H231L
In EGFP, the direction of the transition dipole moment is 14° from the line connecting the centers of the aromatic rings.
Myšková et al. (2020)
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