a.k.a. enhanced GFP, GFPmut1
similar: mEGFP
Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Weak dimer | Aequorea victoria | 26.9 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
488 | 507 | 55,900 | 0.6 | 33.54 | 6.0 | 25.0 | 2.6 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
76.5 ± 6.9 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
76.5 ± 6.9 (10000 cells) | - | HeLa | Shaner et al. (2013) |
76.5 ± 6.9 (10000 cells) | - | HeLa | Hoi et al. (2013) |
- | 3.89 ± 0.25 (50 cells) | U-2 OS | Costantini et al. (2012) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
174.0 | Arc-lamp | Widefield | none | 23.0 | Shaner et al. (2005) | ||
50.1 | 1.5 (mW) | Laser | Point Scanning Confocal | Hela | Zhong et al. (2018) |
EGFP was derived from avGFP with the following mutations: M1_S2insV/F64L/S65T/H231L
No excerpts have been added for EGFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).
(1996). Gene, 173(1) , 33-38. doi: 10.1016/0378-1119(95)00685-0. Article
(2015). Biophysical Journal, 109(2) , 380-389. doi: 10.1016/j.bpj.2015.06.018. Article Pubmed
(2012). PLoS ONE, 7(10) , e47132. doi: 10.1371/journal.pone.0047132. Article Pubmed
(2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article Pubmed
(2001). Chemical Physics, 274(1) , 37-55. doi: 10.1016/s0301-0104(01)00486-4. Article
(1998). Annual Review of Biochemistry, 67(1) , 509-544. doi: 10.1146/annurev.biochem.67.1.509. Article Pubmed
(1998). Methods in Cell Biology, , 19-30. doi: 10.1016/s0091-679x(08)61946-9. Article
(1997). Biophysical Journal, 73(5) , 2782-2790. doi: 10.1016/s0006-3495(97)78307-3. Article
(1996). Nucleic Acids Research, 24(22) , 4592-4593. doi: 10.1093/nar/24.22.4592. Article
(1996). Biochemical and Biophysical Research Communications, 227(3) , 707-711. doi: 10.1006/bbrc.1996.1573. Article Pubmed
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