Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Aequorea victoria | 26.9 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
383 | 448 | 32,000 | 0.56 | 17.92 | 5.3 | 25.0 | 3.0 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
57.0 ± 2.7 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
36.3 (138 cells) | - | HeLa | Costantini et al. (2015) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
55.0 | Subach et al. (2008) | ||||||
635.0 | 3.72 (mW/mm2) | LED | Widefield | Primary hippocampal mouse neurons | 21.0 | Papadaki et al. (2022) | |
1080.0 | 4.25 (mW/mm2) | LED | Widefield | HEK293T | 21.0 | Papadaki et al. (2022) |
EBFP2 was derived from EBFP1.2 with the following mutations: I128V/V150I/D155V/V224R
amino acid numbers relative to avGFP. show relative to EBFP1.2
No excerpts have been added for EBFP2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).
(2007). Biochemistry, 46(20) , 5904-5910. doi: 10.1021/bi700199g. Article Pubmed
(2022). Scientific Reports, 12(1) , 10190. doi: 10.1038/s41598-022-13214-0. Article Pubmed
(2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article Pubmed
(2008). Chemistry & Biology, 15(10) , 1116-1124. doi: 10.1016/j.chembiol.2008.08.006. Article Pubmed
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