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Comparison List

GFP (S65T)

GFP (S65T) is a basic (constitutively fluorescent) green fluorescent protein published in 1995, derived from Aequorea victoria.

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Aggregation Organism Molecular Weight Cofactor
Dimer Aequorea victoria 26.9 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
490 510 55,000 0.64 35.2      

Photostability

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GFP (S65T) Sequence

GFP (S65T) was derived from avGFP with the following mutations: S65T

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for GFP (S65T)
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Improved green fluorescence

Heim R, Cubitt Ab, Tsien Ry

(1995). Nature, 373(6516) , 663-664. doi: 10.1038/373663b0. Article   Pubmed

Additional References

External Resources

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