a.k.a. small Ultra Red Fluorescent Protein
similar: TDsmURFP
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Dimer | Trichodesmium erythraeum IMS101 | 15.0 kDa | Biliverdin |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 642 | 670 | 180,000 | 0.18 | 32.4 | 3.2 | 39.0 | 1.4 |
| t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
|---|---|---|---|---|---|---|---|
| 570.0 | Arc-lamp | Widefield | 37.0 |
smURFP was derived from R3-2+PCB with the following mutations: M1_K2insA/G4S/K9N/S15T/E18K/R33H/F36L/G45S/E48D/Y59F/R61H/D73G/G82S/G96A/K118N/Q129K
In our approach, we first evolved TeAPCα mutants to autocatalytically attach PCB (without a lyase) and fluoresce. Second, since PCB is not present in mammals, we evolved derivatives that bind BV, a molecule ubiquitous in eukaryotes and produced at the rate of 300–500 mg per d in humans.
Rodriguez et al. (2016)
(2016). Nature Methods, 13(9) , 763-769. doi: 10.1038/nmeth.3935. Article Pubmed
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