a.k.a. small Ultra Red Fluorescent Protein
|Dimer||Trichodesmium erythraeum IMS101||15.0 kDa||Biliverdin|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
smURFP was derived from R3-2+PCB with the following mutations: M1_K2insA/G4S/K9N/S15T/E18K/R33H/F36L/G45S/E48D/Y59F/R61H/D73G/G82S/G96A/K118N/Q129K
In our approach, we first evolved TeAPCα mutants to autocatalytically attach PCB (without a lyase) and fluoresce. Second, since PCB is not present in mammals, we evolved derivatives that bind BV, a molecule ubiquitous in eukaryotes and produced at the rate of 300–500 mg per d in humans.