Comparison List


a.k.a. small Ultra Red Fluorescent Protein

similar: TDsmURFP

smURFP is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Trichodesmium erythraeum IMS101. It requires the cofactor biliverdin for fluorescence.
Oligomerization Organism Molecular Weight Cofactor
Dimer Trichodesmium erythraeum IMS101 15.0 kDa Biliverdin


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
642 670 180,000 0.18 32.4      


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
570.0 Arc-lamp Widefield 37.0

smURFP Sequence

smURFP was derived from R3-2+PCB with the following mutations: M1_K2insA/G4S/K9N/S15T/E18K/R33H/F36L/G45S/E48D/Y59F/R61H/D73G/G82S/G96A/K118N/Q129K

GenBank: KX449134


In our approach, we first evolved TeAPCα mutants to autocatalytically attach PCB (without a lyase) and fluoresce. Second, since PCB is not present in mammals, we evolved derivatives that bind BV, a molecule ubiquitous in eukaryotes and produced at the rate of 300–500 mg per d in humans.

Rodriguez et al. (2016)

Primary Reference

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