compare

Comparison List

smURFP

a.k.a. small Ultra Red Fluorescent Protein

similar: TDsmURFP

smURFP is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Trichodesmium erythraeum IMS101. It is reported to be a rapidly-maturing dimer with very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
+
Oligomerization Organism Molecular Weight Cofactor
Dimer Trichodesmium erythraeum IMS101 15.0 kDa Biliverdin

FPbase ID: HGBNQ

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
642 670 180,000 0.18 32.4 3.2 39.0 1.4

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
570.0 Arc-lamp Widefield 37.0

smURFP Sequence

smURFP was derived from R3-2+PCB with the following mutations: M1_K2insA/G4S/K9N/S15T/E18K/R33H/F36L/G45S/E48D/Y59F/R61H/D73G/G82S/G96A/K118N/Q129K

MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAFSQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRCLKEASLSLLDEEDANETAPYFDYIIKAMS
GenBank: KX449134
IPG: ANW47198.1

Structure

Deposited: ,
Chromophore:

Excerpts

In our approach, we first evolved TeAPCα mutants to autocatalytically attach PCB (without a lyase) and fluoresce. Second, since PCB is not present in mammals, we evolved derivatives that bind BV, a molecule ubiquitous in eukaryotes and produced at the rate of 300–500 mg per d in humans.

Rodriguez et al. (2016)

Primary Reference

Additional References

    No additional references have been added.

External Resources

Change history

Something missing or incorrect? Submit a change Submit a change