(2012). Nature Methods, 9(10) , 1005-1012. doi: 10.1038/nmeth.2171. Article Pubmed
With a completely deprotonated chromophore at neutral pH (pKa = 6.1), Clover should be incapable of photoconversion despite the presence of His203, which enhances photoconversion in PA-GFP. Indeed, we observed no photoactivation of Clover in illumination conditions that produced more than threefold photoactivation of PA-GFP.
The precursor to mRuby, eqFP611 dimer, readily undergoes reversible photobleaching due to isomerization from a bright trans form to a dim cis form, and mutations near the chromophore affect isomerization