Microscopes on FPbase are collections of
configurations, including filters, light sources, lasers, and
detectors, meant to represent the hardware of a specific microscope or other imaging device. Once you save your
microscope configuration, you get a personalized spectra-viewer app that you can use to visualize the efficiency of
excitation, collection, and predicted brightness of any of the fluorophores in FPbase for your hardware. (As a bonus,
you can also quickly recall the filter settings from any of your optical configs in the general FPbase spectra viewer) You can
create as many different microscope pages as you'd like and you can share the link to your microscope page with
anyone, or embed the app in your website for others to use.
For more information on building a microscope page, see the
Fluorophore Efficiency Reports
Once you have saved the components of your microscope, you can generate a "fluorophore efficiency report": a
prediction for excitation and collection efficiency for every fluorophore in the database for which spectral data is
available, matched with every optical configuration in your scope. Either click the icon next to the corresponding microscope here, or find the report button at
the bottom of any microscope page.