Microscopes on FPbase are collections of optical configurations, including filters, light sources, lasers, and detectors, meant to represent the hardware of a specific microscope or other imaging device. Once you save your microscope configuration, you get a personalized spectra-viewer app that you can use to visualize the efficiency of excitation, collection, and predicted brightness of any of the fluorophores in FPbase for your hardware. You can create as many different microscope pages as you'd like and you can share the link to your microscope page with anyone, or embed the app in your website for others to use.
Fluorophore Efficiency Reports
Once you have saved the components of your microscope, you can generate a "fluorophore efficiency report": a prediction for excitation and collection efficiency for every fluorophore in the database for which spectral data is available, matched with every optical configuration in your scope. Either click the icon next to the corresponding microscope here, or find the report button at the bottom of any microscope page.
TESMTIRF Epifluorescence Structured-Light Microscope – Jun 14, 2019
Vanderbilt Lattice Light Sheet MicroscopeAll available filter combinations for single, dual, and multichannel fluorescence on our LLSM. Four available laser lines (488, 561, 583, 640nm). SNAP tagged JF dyes available upon request. – Jun 12, 2019
Vanderbilt MANTISVanderbilt MANTIS can do CARS, SRS, Multiphoton, and SHG imaging, all with ultrafast, nonlinear excitation. – Jun 12, 2019
Vanderbilt SOPiVanderbilt Scanning Oblique Plane Illumination (SOPi) Single Objective Lightsheet Microscope. Laser Lines: 488, 561, 640. Photometrics Prime 95B. 0.34NA effectively – Jun 12, 2019
SpinsterYokogawa CSU-W1 Spinning Disk on Nikon T1 – May 28, 2019
Lil'NickyNikon Ti widefield and TIRF microscope – May 28, 2019
Vecchiarelli Lab - Ti2Ti2-E, with wide-field fluorescence (Filters for Phase Contrast, DIC, DAPI, CFP, GFP, YFP, TxRed, CY5) and motorized TIRF capabilities with 3 Excitation lasers (488, 561, 633nm), autofocus, XY-mini-scanner for 405nm FRAP and photo-activation, a Prime95b CMOS camera, and NIS elements acquisition and image processing software on a dedicated computer. – May 24, 2019