|Monomer||Aequorea victoria||26.9 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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We had initially presumed that EBFP was close to the maximum achievable fluorescent brightness for its particular chromophore structure. However, the recent report from Waldo and co-workers that introduction of the “superfolder” mutations into BFP improved the fluorescent brightness in bacterial colonies galvanized us to explore whether these mutations could also benefit EBFP. Mutations S30R/Y39N/T65S/S72A/N105T/I171V/N198S/A206V were introduced into EBFP by site-directed mutagenesis to produce EBFP1.2