a.k.a. TDimer2
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Tandem dimer | Discosoma sp. | 52.7 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 552 | 579 | 120,000 | 0.68 | 81.6 | 4.8 | 120.0 |
No photostability measurements available ... add one!
Based on our early results, it seemed as though engineering a true monomer of DsRed might be impossible and therefore we pursued an alternate approach. The basic strategy was to fuse two copies of our best AC dimer with a polypeptide linker such that the critical dimer interactions could be satisfied through intramolecular contacts with the tandem partner encoded within the same polypeptide. With our optimized dimer2 we constructed a series of tandem constructs with linkers of varying lengths (9, 12, 13, or 22 aa) and a sequence similar to a known protease-resistant linker. Of these four, only the tandem construct with the nine-residue linker was notable for its somewhat slower maturation. The other three constructs were practically indistinguishable, and the tandem construct with the 12-residue linker, designated tdimer2(12), is currently our preferred construct because it has the shortest linker.
Campbell et al. (2002)
(2002). Proceedings of the National Academy of Sciences, 99(12) , 7877-7882. doi: 10.1073/pnas.082243699. Article Pubmed
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