A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

Nagai T, Ibata K, Park Es, Kubota M, Mikoshiba K, Miyawaki A

(2002). Nature Biotechnology, 20(1) , 87-90. doi: 10.1038/nbt0102-87. Article   Pubmed

    Primary Proteins:
  1. EYFP-F46L
  2. SEYFP
  3. Venus
Add photostability measurements

Excerpts

F46L accelerates the oxidation step, leading to the enhancement of fluorescence development of YFP at 37°C. The SEYFP-F46L variant folds and forms the chromophore very efficiently at 37°C.