Age-dependent Preferential Dense-Core Vesicle Exocytosis in Neuroendocrine Cells Revealed by Newly Developed Monomeric Fluorescent Timer Protein

Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A

(2010). Molecular Biology of the Cell, 21(1) , 87-94. doi: 10.1091/mbc.e09-08-0722. Article   Pubmed

    Primary Proteins:
  1. mK-GO
    Secondary Proteins:
  1. DsRed-Timer
Add photostability measurements

Excerpts

The ratio of orange per green fluorescence was lineally increased and reached at plateau at ∼10 h. The time for the half-maximal fluorescence development is ∼6 h which is faster than DsRed-based fluorescent timer

mK-GO is a monomeric version of fluorescent timer that is suitable for monitoring the age of the targeting protein.