Takeharu Nagai

1. Circularly permuted green fluorescent proteins engineered to sense Ca2+

   Nagai T, Sawano A, Park Es, Miyawaki A

   (2001). Proceedings of the National Academy of Sciences, 98(6) , 3197-3202. doi: 10.1073/pnas.051636098. Article   Pubmed

   Primary Proteins:

   1. cpEYFP(V68L/Q69K)

2. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications

   Nagai T, Ibata K, Park Es, Kubota M, Mikoshiba K, Miyawaki A

   (2002). Nature Biotechnology, 20(1) , 87-90. doi: 10.1038/nbt0102-87. Article   Pubmed

   Primary Proteins:

   1. EYFP-F46L
   2. SEYFP
   3. Venus

3. Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity

   Rekas A, Alattia J-R, Nagai T, Miyawaki A, Ikura M

   (2002). Journal of Biological Chemistry, 277(52) , 50573-50578. doi: 10.1074/jbc.m209524200. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Venus

4. An ultramarine fluorescent protein with increased photostability and pH insensitivity

   Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T

   (2009). Nature Methods, 6(5) , 351-353. doi: 10.1038/nmeth.1317. Article   Pubmed

   Primary Proteins:

   1. Sirius

5. Highlighted Ca2+ imaging with a genetically encoded ‘caged’ indicator

   Matsuda T, Horikawa K, Saito K, Nagai T

   (2013). Scientific Reports, 3(1) , 1398. doi: 10.1038/srep01398. Article   Pubmed

   Primary Proteins:

   1. DarkVenus
   2. DimVenus

6. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T

   (2013). Scientific Reports, 3(1) , 2629. doi: 10.1038/srep02629. Article   Pubmed

   Primary Proteins:

   1. SuperNova Red

7. A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy

   Tiwari Dk, Arai Y, Yamanaka M, Matsuda T, Agetsuma M, Nakano M, Fujita K, Nagai T

   (2015). Nature Methods, 12(6) , 515-518. doi: 10.1038/nmeth.3362. Article   Pubmed

   Primary Proteins:

   1. Kohinoor

8. Red fluorescent cAMP indicator with increased affinity and expanded dynamic range

   Ohta Y, Furuta T, Nagai T, Horikawa K

   (2018). Scientific Reports, 8(1) , 1866. doi: 10.1038/s41598-018-20251-1. Article   Pubmed

   Primary Proteins:

   1. R-FlincA

9. Acid-Tolerant Monomeric GFP from Olindias formosa

   Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T

   (2018). Cell Chemical Biology, 25(3) , 330-338.e7. doi: 10.1016/j.chembiol.2017.12.005. Article   Pubmed

   Primary Proteins:

   1. dfGFP
   2. Gamillus
   3. Gamillus0.1
   4. Gamillus0.2
   5. Gamillus0.3
   6. Gamillus0.4
   7. Gamillus0.5

10. Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation

    Riani Yd, Matsuda T, Takemoto K, Nagai T

    (2018). BMC Biology, 16(1) , 50. doi: 10.1186/s12915-018-0514-7. Article   Pubmed

    Primary Proteins:

    1. SuperNova Green

11. A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging

    Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T

    (2018). ACS Chemical Biology, , . doi: 10.1021/acschembio.8b00200. Article   Pubmed

    Primary Proteins:

    1. SPOON

12. A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging

    Wazawa T, Noma R, Uto S, Sugiura K, Washio T, Nagai T

    (2021). Microscopy, , . doi: 10.1093/jmicro/dfab001. Article   Pubmed

    Primary Proteins:

    1. Kohinoor2.0

13. Structure‐based analysis and evolution of a monomerized red‐colored chromoprotein from the <i>Olindias formosa</i> jellyfish

    Zhai L, Nakashima R, Shinoda H, Ike Y, Matsuda T, Nagai T

    (2022). Protein Science, 31(5) , e4285. doi: 10.1002/pro.4285. Article   Pubmed

    Primary Proteins:

    1. M-Velour-537
    2. M-Velour-553
    3. M-Velour-554
    4. ofCP
    5. O-Velour
    6. R-Velour

14. Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application

    Sugiura K, Nagai T

    (2022). Communications Biology, 5(1) , 1172. doi: 10.1038/s42003-022-04153-7. Article   Pubmed

    Primary Proteins:

    1. Sumire