Comparison List


td5oxStayGold is a basic (constitutively fluorescent) green fluorescent protein published in 2023, derived from Cytaeis uchidae.

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Oligomerization Organism Molecular Weight Cofactor
Tandem dimer Cytaeis uchidae 54.4 kDa -

FPbase ID: JVB9K


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
496 506 156,000 0.93 145.08      


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td5oxStayGold Sequence

td5oxStayGold was derived from tdoxStayGold with the following mutations: E173c_G173wdel/S173yH/S173aT/A173b_G173gdel/S173k_G173cdel/A173h_G173jdel/S173mT/G173o_G173ldelinsS/A173nE/G173oD/G173pN/K173qN/L173rI/L173r_M173sinsD

GenBank: LC756336


To improve the dispersibility of tdoxStayGold, we re-examined the polypeptide linker between the two copies of (ox)StayGold. First, we trimmed the EV linker (116 residues) into three polypeptides that spanned 34, 66 and 97 amino acids; however, none of the td constructs with these shortened EV linkers (td2oxStayGold, td3oxStayGold and td4oxStayGold, respectively) showed improved OSER or Fluoppi scores. We subsequently harnessed the linker used for generating tdTomato. The use of a 21-residue linker resulted in the generation of td5StayGold and td5oxStayGold, both of which gave high OSER scores (91.5% and 88.5%, respectively) and high Fluoppi scores (96.4% and 94.1%, respectively).

Ando et al. (2023)

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