Virgile Adam

1. Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations

   Adam V, Lelimousin M, Boehme S, Desfonds G, Nienhaus K, Field Mj, Wiedenmann J, Mcsweeney S, Nienhaus Gu, Bourgeois D

   (2008). Proceedings of the National Academy of Sciences, 105(47) , 18343-18348. doi: 10.1073/pnas.0805949105. Article   Pubmed

   Primary Proteins:

   1. IrisFP

2. Structural Basis of Enhanced Photoconversion Yield in Green Fluorescent Protein-like Protein Dendra2

   Adam V, Nienhaus K, Bourgeois D, Nienhaus Gu

   (2009). Biochemistry, 48(22) , 4905-4915. doi: 10.1021/bi900383a. Article   Pubmed

   Primary Proteins:

3. Data storage based on photochromic and photoconvertible fluorescent proteins

   Adam V, Mizuno H, Grichine A, Hotta J-, Yamagata Y, Moeyaert B, Nienhaus Gu, Miyawaki A, Bourgeois D, Hofkens J

   (2010). Journal of Biotechnology, 149(4) , 289-298. doi: 10.1016/j.jbiotec.2010.04.001. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. IrisFP

4. From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family

   Wiedenmann J, Gayda S, Adam V, Oswald F, Nienhaus K, Bourgeois D, Nienhaus Gu

   (2011). Journal of Biophotonics, 4(6) , 377-390. doi: 10.1002/jbio.201000122. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. IrisFP

5. Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications

   Adam V, Moeyaert B, David Cc, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J

   (2011). Chemistry & Biology, 18(10) , 1241-1251. doi: 10.1016/j.chembiol.2011.08.007. Article   Pubmed

   Primary Proteins:

   1. Dendra2-M159A
   2. mEosFP-F173S
   3. mEosFP-M159A
   4. NijiFP

   Additional Proteins:

   1. Dendra2
   2. mEosFP

6. The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein

   Roy A, Field Mj, Adam V, Bourgeois D

   (2011). Journal of the American Chemical Society, 133(46) , 18586-18589. doi: 10.1021/ja2085355. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. IrisFP

7. Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2

   Fron E, Van Der Auweraer M, Moeyaert B, Michiels J, Mizuno H, Hofkens J, Adam V

   (2013). The Journal of Physical Chemistry B, 117(8) , 2300-2313. doi: 10.1021/jp309219m. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Dendra2

8. Structural Evidence for a Two-Regime Photobleaching Mechanism in a Reversibly Switchable Fluorescent Protein

   Duan C, Adam V, Byrdin M, Ridard J, Kieffer-Jaquinod S, Morlot C, Arcizet D, Demachy I, Bourgeois D

   (2013). Journal of the American Chemical Society, 135(42) , 15841-15850. doi: 10.1021/ja406860e. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. IrisFP

9. Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy

   Fron E, Sliwa M, Adam V, Michiels J, Rocha S, Dedecker P, Hofkens J, Mizuno H

   (2014). Photochem. Photobiol. Sci., 13(6) , 867-874. doi: 10.1039/c3pp50335f. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Kaede

10. Rational design of enhanced photoresistance in a photoswitchable fluorescent protein

    Duan C, Byrdin M, El Khatib M, Henry X, Adam V, Bourgeois D

    (2015). Methods and Applications in Fluorescence, 3(1) , 014004. doi: 10.1088/2050-6120/3/1/014004. Article   Pubmed

    Primary Proteins:

    1. IrisFP-M159A

    Additional Proteins:

    1. IrisFP

11. Arginine 66 Controls Dark-State Formation in Green-to-Red Photoconvertible Fluorescent Proteins

    Berardozzi R, Adam V, Martins A, Bourgeois D

    (2016). Journal of the American Chemical Society, 138(2) , 558-565. doi: 10.1021/jacs.5b09923. Article   Pubmed

    Primary Proteins:

    1. Dendra2-T69A
    2. mEos2-A69T

    Additional Proteins:

    1. mEos2

12. Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm

    El Khatib M, Martins A, Bourgeois D, Colletier J-P, Adam V

    (2016). Scientific Reports, 6(1) , 18459. doi: 10.1038/srep18459. Article   Pubmed

    Primary Proteins:

    1. rsFolder
    2. rsFolder2

    Additional Proteins:

    1. rsEGFP2

13. Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP

    Colletier J-P, Sliwa M, Gallat F-X, Sugahara M, Guillon V, Schirò G, Coquelle N, Woodhouse J, Roux L, Gotthard G, Royant A, Uriarte Lm, Ruckebusch C, Joti Y, Byrdin M, Mizohata E, Nango E, Tanaka T, Tono K, Yabashi M, Adam V, Cammarata M, Schlichting I, Bourgeois D, Weik M

    (2016). The Journal of Physical Chemistry Letters, 7(5) , 882-887. doi: 10.1021/acs.jpclett.5b02789. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. IrisFP

14. Photoswitching of Green mEos2 by Intense 561 nm Light Perturbs Efficient Green-to-Red Photoconversion in Localization Microscopy

    Thédié D, Berardozzi R, Adam V, Bourgeois D

    (2017). The Journal of Physical Chemistry Letters, 8(18) , 4424-4430. doi: 10.1021/acs.jpclett.7b01701. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. mEos2

15. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

    De Zitter E, Thédié D, Mönkemöller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D

    (2019). Nature Methods, , . doi: 10.1038/s41592-019-0462-3. Article

    Primary Proteins:

    Additional Proteins:

    1. mEos4b

16. Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

    Woodhouse J, Nass Kovacs G, Coquelle N, Uriarte Lm, Adam V, Barends Trm, Byrdin M, De La Mora E, Bruce Doak R, Feliks M, Field M, Fieschi F, Guillon V, Jakobs S, Joti Y, Macheboeuf P, Motomura K, Nass K, Owada S, Roome Cm, Ruckebusch C, Schirò G, Shoeman Rl, Thepaut M, Togashi T, Tono K, Yabashi M, Cammarata M, Foucar L, Bourgeois D, Sliwa M, Colletier J-P, Schlichting I, Weik M

    (2020). Nature Communications, 11(1) , 741. doi: 10.1038/s41467-020-14537-0. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. rsEGFP2

17. Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State

    De Zitter E, Ridard J, Thédié D, Adam V, Lévy B, Byrdin M, Gotthard G, Van Meervelt L, Dedecker P, Demachy I, Bourgeois D

    (2020). Journal of the American Chemical Society, 142(25) , 10978-10988. doi: 10.1021/jacs.0c01880. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. mEos4b

18. Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement**

    Adam V, Hadjidemetriou K, Jensen N, Shoeman Rl, Woodhouse J, Aquila A, Banneville As, Barends Trm, Bezchastnov V, Boutet S, Byrdin M, Cammarata M, Carbajo S, Eleni Christou N, Coquelle N, De La Mora E, El Khatib M, Moreno Chicano T, Bruce Doak R, Fieschi F, Foucar L, Glushonkov O, Gorel A, Grünbein Ml, Hilpert M, Hunter M, Kloos M, Koglin Je, Lane Tj, Liang M, Mantovanelli A, Nass K, Nass Kovacs G, Owada S, Roome Cm, Schirò G, Seaberg M, Stricker M, Thépaut M, Tono K, Ueda K, Uriarte Lm, You D, Zala N, Domratcheva T, Jakobs S, Sliwa M, Schlichting I, Colletier Jp, Bourgeois D, Weik M

    (2022). ChemPhysChem, 23(19) , e202200192. doi: 10.1002/cphc.202200192. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. rsEGFP2

19. Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2

    Mantovanelli Amr, Glushonkov O, Adam V, Wulffelé J, Thédié D, Byrdin M, Gregor I, Nevskyi O, Enderlein J, Bourgeois D

    (2023). Journal of the American Chemical Society, 145(27) , 14636-14646. doi: 10.1021/jacs.3c01500. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. rsEGFP2

20. Structural Heterogeneity in a Phototransformable Fluorescent Protein Impacts its Photochemical Properties

    Maity A, Wulffelé J, Ayala I, Favier A, Adam V, Bourgeois D, Brutscher B

    (2023). Advanced Science, 11(10) , e2306272. doi: 10.1002/advs.202306272. Article   Pubmed

    Primary Proteins:

    Additional Proteins:

    1. mEos4b