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Comparison List

IrisFP

similar: mIrisFP

IrisFP is a multi-photochromic orange fluorescent protein published in 2008, derived from Lobophyllia hemprichii.

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Oligomerization Organism Molecular Weight Cofactor
Tetramer Lobophyllia hemprichii 25.7 kDa -

FPbase ID: PA6OT

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Orange (off)              
Green (off)              
Orange 551 580 35,400 0.47 16.64      
Green 488 516 52,200 0.43 22.45 5.7    

Transitions

From To Switch λ
Green (off) Green 405
Green Orange 405
Orange (off) Orange 440
Green Green (off) 488
Orange Orange (off) 561

Photostability

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IrisFP Sequence

IrisFP was derived from EosFP with the following mutations: F173S/F191L

MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALLLEGNAHYRCDSRTTYKAKEKGVKLPGYHLVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for IrisFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. Rational design of enhanced photoresistance in a photoswitchable fluorescent protein

    Duan C, Byrdin M, El Khatib M, Henry X, Adam V, Bourgeois D

    (2015). Methods and Applications in Fluorescence, 3(1) , 014004. doi: 10.1088/2050-6120/3/1/014004. Article   Pubmed

  2. The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein

    Roy A, Field Mj, Adam V, Bourgeois D

    (2011). Journal of the American Chemical Society, 133(46) , 18586-18589. doi: 10.1021/ja2085355. Article   Pubmed

External Resources

Change history

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