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| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Dimer | Cytaeis uchidae | 24.6 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 496 | 505 |
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C. uchidae (phylum Cnidaria, class Hydrozoa) produces colonies of fluorescent polyps on shells of Nassarius livescens, a gastropod living in the sandy-mud bottom of the sea. It also produces millimeter-sized free-swimming medusae that express green fluorescence in the epithelium of the ex-umbrella and the sub-umbrella, as well as in the gonads. Our RNA sequencing and subsequent anchored PCR analyses using total RNA from C. uchidae identified a transcript encoding an FP with a chromophore-forming tripeptide GYG at the appropriate position. This protein [is] referred to as CU17S.
Hirano et al. (2022)
CU17S has a unique primary structure. The closest homologue, sharing only 15.2% identity, was obeCFP, an FP cloned from Obelia medusa (Aglyamova et al. 2011)
Hirano et al. (2022)
(2022). Nature Biotechnology, , . doi: 10.1038/s41587-022-01278-2. Article Pubmed
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