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Comparison List

CU17S

CU17S is a basic (constitutively fluorescent) green fluorescent protein published in 2022, derived from Cytaeis uchidae.

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Oligomerization Organism Molecular Weight Cofactor
Dimer Cytaeis uchidae 24.6 kDa -

FPbase ID: 5DXHD

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
496 505          

Photostability

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CU17S Sequence

MASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTLLYPLLSDKSKCVEVHQNTICKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHICQSETLEAHL
GenBank: LC593677

Excerpts

C. uchidae (phylum Cnidaria, class Hydrozoa) produces colonies of fluorescent polyps on shells of Nassarius livescens, a gastropod living in the sandy-mud bottom of the sea. It also produces millimeter-sized free-swimming medusae that express green fluorescence in the epithelium of the ex-umbrella and the sub-umbrella, as well as in the gonads. Our RNA sequencing and subsequent anchored PCR analyses using total RNA from C. uchidae identified a transcript encoding an FP with a chromophore-forming tripeptide GYG at the appropriate position. This protein [is] referred to as CU17S.

Hirano et al. (2022)

CU17S has a unique primary structure. The closest homologue, sharing only 15.2% identity, was obeCFP, an FP cloned from Obelia medusa (Aglyamova et al. 2011)

Hirano et al. (2022)

Primary Reference

Additional References

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External Resources

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