similar: StayGold
No spectrum has been submitted ... add a spectrum!
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Tandem dimer | Cytaeis uchidae | 60.8 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 496 | 504 | 162,000 | 0.9 | 145.8 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 0.922 (2326 cells) | - | Hela | Hirano et al. (2022) |
No photostability measurements available ... add one!
tdStayGold was derived from (n1)StayGold with the following mutations: *217_M1insMVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTLLYPLLSDKSKCVEAHQNTICKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHICQSETLEAHLPWHEPSASAVEFSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGKL
For protein fusion applications, we attempted to fuse two copies of a StayGold construct that was appended at both the N- and C-termini using a flexible linker (EV linker)29 to create a tandem dimer. The resultant FP construct, tdStayGold, showed the same photostability as StayGold. An OSER assay3 using CytERM-tdStayGold showed almost no whorl structures, indicating the monovalent fusion of tdStayGold.
Hirano et al. (2022)
(2022). Nature Biotechnology, , . doi: 10.1038/s41587-022-01278-2. Article Pubmed
Something missing or incorrect? Submit a change Submit a change