a.k.a. mKusabira Green Orange
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Verrillofungia concinna | 24.5 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| Late | 548 | 561 | 42,000 | 4.8 | 360.0 | |||
| Early | 500 | 509 | 35,900 | 6.0 |
No photostability measurements available ... add one!
mK-GO was derived from mKO with the following mutations: K48E/P69V/K184E/K187E/S191D/S195G
amino acid numbers relative to KO. show relative to mKO
The ratio of orange per green fluorescence was lineally increased and reached at plateau at ∼10 h. The time for the half-maximal fluorescence development is ∼6 h which is faster than DsRed-based fluorescent timer
Tsuboi et al. (2009)
mK-GO is a monomeric version of fluorescent timer that is suitable for monitoring the age of the targeting protein.
Tsuboi et al. (2009)
(2010). Molecular Biology of the Cell, 21(1) , 87-94. doi: 10.1091/mbc.e09-08-0722. Article Pubmed
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