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Comparison List

mK-GO

a.k.a. mKusabira Green Orange

mK-GO is a multistate orange fluorescent protein published in 2010, derived from Verrillofungia concinna. It is reported to be a very slowly-maturing monomer with low acid sensitivity.

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Aggregation Organism Molecular Weight Cofactor
Monomer Verrillofungia concinna 24.5 kDa -

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Late 548 561 42,000     4.8 360.0  
Early 500 509 35,900     6.0    
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Photostability

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mK-GO Sequence

mK-GO was derived from mKO with the following mutations: K48E/P69V/K184E/K187E/S191D/S195G
amino acid numbers relative to KO. show relative to mKO

MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITELVEDAVAHS

Excerpts

The ratio of orange per green fluorescence was lineally increased and reached at plateau at ∼10 h. The time for the half-maximal fluorescence development is ∼6 h which is faster than DsRed-based fluorescent timer

Tsuboi et al. (2009)

mK-GO is a monomeric version of fluorescent timer that is suitable for monitoring the age of the targeting protein.

Tsuboi et al. (2009)

Primary Reference

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