Comparison List


a.k.a. (n1)SG

(n1)StayGold is a basic (constitutively fluorescent) green fluorescent protein published in 2022, derived from Cytaeis uchidae.

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Oligomerization Organism Molecular Weight Cofactor
Dimer Cytaeis uchidae 25.6 kDa -

FPbase ID: 4Y1D1


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
496 505          


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(n1)StayGold Sequence

(n1)StayGold was derived from StayGold with the following mutations: A2V/T4_P5insGEELFTGVV



StayGold’s head and tail are relatively short, which makes this FP sensitive to C- and N-terminal fusions. We borrowed termini from other proven FPs and found that nine amino acids from the N-terminal region of EGFP (n1 or n2) and ten amino acids at the C-terminus of dfGFP (c4) could be fused to StayGold to improve its targeting function to some subcellular components.

Hirano et al. (2022)

Primary Reference

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