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Comparison List

mKeima

mKeima is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2006, derived from Montipora sp. 20. It has high acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Montipora sp. 20 25.1 kDa -

FPbase ID: SXBT1

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
pH5 586 620     6.5    
pH6 440 620     6.5    
pH8 440 620     6.5    
pH4 586 620     6.5    
pH7.4 440 620 14,400 0.24 3.46 6.5 180.0 2.93
Edit state transitions

Photostability

No photostability measurements available ... add one!

mKeima Sequence

mKeima was derived from dKeima with the following mutations: L60Q/F61L/V79F/T92S/T123E/Y188R/Y190E
amino acid numbers relative to Montipora sp. #20. show relative to dKeima

MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVTKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTVSNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKLEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
GenBank: BAE93226
UniProtKB: Q1JV70
IPG: 5593672

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mKeima
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. mBeRFP, an Improved Large Stokes Shift Red Fluorescent Protein

    Yang J, Wang L, Yang F, Luo H, Xu L, Lu J, Zeng S, Zhang Z

    (2013). PLoS ONE, 8(6) , e64849. doi: 10.1371/journal.pone.0064849. Article   Pubmed

  2. Excited State Proton Transfer in the Red Fluorescent Protein mKeima

    Henderson Jn, Osborn Mf, Koon N, Gepshtein R, Huppert D, Remington Sj

    (2009). Journal of the American Chemical Society, 131(37) , 13212-13213. doi: 10.1021/ja904665x. Article   Pubmed

External Resources

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