compare

Comparison List

DsRed-Timer

a.k.a. Timer, DsRed-E5, E5

DsRed-Timer is a fluorescent protein published in 2000, derived from Discosoma sp.. It is reported to be a tetramer.

No spectrum has been submitted ... add a spectrum!

Aggregation Organism Molecular Weight Cofactor
Tetramer Discosoma sp. 25.9 kDa -

Attributes

This protein does not yet have any fluorescent states assigned. Submit a change.

Photostability

no photostability measurements available ... add one!

DsRed-Timer Sequence

DsRed-Timer was derived from DsRed with the following mutations: V105A/S197T

MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRHHLFL

Excerpts

The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression.

Terskikh (2000)

Primary Reference

"Fluorescent Timer": Protein That Changes Color with Time

Terskikh A

(2000). Science, 290(5496) , 1585-1588. doi: 10.1126/science.290.5496.1585. Article

Additional References

  1. Use of the Fluorescent Timer DsRED-E5 as Reporter to Monitor Dynamics of Gene Activity in Plants

    Mirabella R

    (2004). PLANT PHYSIOLOGY, 135(4) , 1879-1887. doi: 10.1104/pp.103.038539. Article   Pubmed

External Resources

Something missing or incorrect? Submit a change Submit a change