PDM1-4 is a fluorescent protein published in 2010, derived from Echinophyllia sp. SC22. It is reported to be a tetramer.
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PDM1-4 was derived from
Dronpa with the following mutations: K145N
An attempt to find
Dronpa variants with slower off-switching rate has led to the identification of PDM1-4. Unfortunately, PDM1-4 was prone to form tetramers and did not show improvement in photon output. In addition, PDM1-4 has worsened photostability and less contrast between on and off-states.
Chang et al. (2012)
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Photochemical & Photobiological Sciences, 9(2) , 239. doi: 10.1039/b9pp00124g.
Acta Crystallographica Section D Biological Crystallography, 68(12) , 1653-1659. doi: 10.1107/s0907444912039686.
Proceedings of the National Academy of Sciences, 109(12) , 4455-4460. doi: 10.1073/pnas.1113770109.
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