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Comparison List

dKeima570

dKeima570 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2006, derived from Montipora sp. 20.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Montipora sp. 20 25.0 kDa -

FPbase ID: BUJNN

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
440 570 14,000 0.15 2.1      

Photostability

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dKeima570 Sequence

dKeima570 was derived from dKeima with the following mutations: F61M/Q62C
amino acid numbers relative to Montipora sp. #20. show relative to dKeima

Note: Note: there is inconsistency between the dKeima570 sequence reported in the text and in Supp. Fig. 1 of Kogure et al 2006 (which reports dKeima570 as dKeima + F61M/Q62C) and the GenBank BAE94262 sequence, which reverts isoleucine 191 back to valine. Here we use the sequence reported in the paper.

MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVTKGGPLPFAWDILSPLMCYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTVSNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKLEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
GenBank: BAE94262
UniProtKB: Q1JU63
IPG: 5680967

Excerpts

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Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

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