Quantitative assessment of fluorescent proteins

Cranfill Pj, Sell Br, Baird Ma, Allen Jr, Lavagnino Z, De Gruiter Hm, Kremers G-J, Davidson Mw, Ustione A, Piston Dw

(2016). Nature Methods, 13(7) , 557-562. doi: 10.1038/nmeth.3891. Article   Pubmed

    Secondary Proteins:
  1. mEGFP,
  2. mEos4a,
  3. mEos4b,
  4. mTagBFP2

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mEGFP 46.0 240.0 uW Laser Point Scanning Confocal
mEGFP 239.0 60.0 uW Laser Point Scanning Confocal
Superfolder GFP 208.26 80.0 uW Laser idk
A caution on interpretation of photostability measurements
Add photostability measurements

OSER Measurements

Protein % Normal Cells OSER/NE ratio Cell Type Temp (˚C)
mEGFP 98.1 ± 1.6 (10000 cells) - HeLa -
mEmerald 96.6 ± 1.1 (10000 cells) - HeLa -
mRFP1 95.8 ± 1.1 (10000 cells) - HeLa -
mT-Sapphire 95.5 ± 0.6 (10000 cells) - HeLa -
mApple 95.3 ± 1.7 (10000 cells) - HeLa -
mPapaya 95.1 ± 1.1 (10000 cells) - HeLa -
CyPet 94.0 ± 2.4 (10000 cells) - HeLa -
mCitrine 93.8 ± 2.6 (10000 cells) - HeLa -
mTurquoise2 93.8 ± 1.0 (10000 cells) - HeLa -
mTurquoise 93.3 ± 1.2 (10000 cells) - HeLa -
mRuby 93.1 ± 2.1 (10000 cells) - HeLa -
mCerulean 92.6 ± 2.0 (10000 cells) - HeLa -
mTFP1 92.0 ± 1.5 (10000 cells) - HeLa -
Topaz 91.9 ± 2.0 (10000 cells) - HeLa -
mOrange2 91.8 ± 1.4 (10000 cells) - HeLa -
FusionRed 91.5 ± 3.0 (10000 cells) - HeLa -
mPlum 91.5 ± 2.4 (10000 cells) - HeLa -
mCerulean3 91.0 ± 1.8 (10000 cells) - HeLa -
mStrawberry 90.6 ± 2.5 (10000 cells) - HeLa -
mAzamiGreen 90.3 ± 4.7 (10000 cells) - HeLa -
mAmetrine 90.0 ± 4.5 (10000 cells) - HeLa -
mRaspberry 89.6 ± 4.0 (10000 cells) - HeLa -
mRuby2 87.4 ± 5.8 (10000 cells) - HeLa -
mWasabi 86.4 ± 2.5 (10000 cells) - HeLa -
mVenus 83.9 ± 4.4 (10000 cells) - HeLa -
mKate2 81.1 ± 6.1 (10000 cells) - HeLa -
Cerulean 78.3 ± 4.8 (10000 cells) - HeLa -
Clover 72.9 ± 5.5 (10000 cells) - HeLa -
mKO2 68.4 ± 4.2 (10000 cells) - HeLa -
Superfolder GFP 62.4 ± 6.3 (10000 cells) - HeLa -
TagRFP 57.7 ± 7.5 (10000 cells) - HeLa -
tdTomato 57.6 ± 4.9 (10000 cells) - HeLa -
EBFP2 57.0 ± 2.7 (10000 cells) - HeLa -
mKate 54.5 ± 4.8 (10000 cells) - HeLa -
mTagBFP2 49.8 ± 1.9 (10000 cells) - HeLa -
mKO 46.9 ± 6.1 (10000 cells) - HeLa -
MiCy 41.7 ± 10.7 (10000 cells) - HeLa -
mCardinal 41.3 ± 3.6 (10000 cells) - HeLa -
TagRFP-T 41.2 ± 4.0 (10000 cells) - HeLa -
mNeptune 40.5 ± 3.8 (10000 cells) - HeLa -
Venus 36.5 ± 3.5 (10000 cells) - HeLa -
Citrine 36.2 ± 3.9 (10000 cells) - HeLa -
TurboRFP - (10000 cells) - HeLa -
Katushka - (10000 cells) - HeLa -
DsRed2 - (10000 cells) - HeLa -
YPet 62.5 ± 5.2 (10000 cells) - HeLa -
mOrange 91.4 ± 1.7 (10000 cells) - HeLa -
mCherry 95.0 ± 0.8 (10000 cells) - HeLa -
mNeonGreen 90.4 ± 2.1 (10000 cells) - HeLa -
EGFP 76.5 ± 6.9 (10000 cells) - HeLa -

Excerpts

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