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YPet was derived from EYFP with the following mutations: F47L/I48L/F65L/L69V/M154T/V164A/S176G/S209F/V225L/L232E/D235NF46L/I47L/F64L/L68V/M153T/V163A/S175G/S208F/V224L/L231E/D234N
amino acid numbers relative to EYFPavGFP. show relative to avGFPshow relative to EYFP
Nguyen and Daugherty (2005) reported the development of an improved FRET pair, YPet & CyPet, which had a sevenfold enhancement of the FRET signal. However, we discovered that most of the signal enhancement was due to enhanced dimerization of YPet to CyPet within the tethered construct, and was substantially reduced when we incorporated the monomerizing mutation previously developed to prevent the weak dimerization of GFP (Zacharias et al. 2002).