Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Aequorea victoria | 26.9 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
434 | 474 | 30,000 | 0.93 | 27.9 | 3.1 | 33.5 | 4.0 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
93.8 ± 1.0 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
88.0 | - | - | Meiresonne et al. (2019) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
90.0 | Goedhart et al. (2012) |
mTurquoise2 was derived from mTurquoise with the following mutations: I146F
amino acid numbers relative to avGFP. show relative to mTurquoise
Structural analysis of mTurquoise unveiled one suboptimal residue, Ile146, which was targeted for further improvement... The X-ray structure of the the I146F mutant, dubbed mTurquoise2, reveals that the mutated residue contributes to an improved packing of the chromophore through many further van der Waals interactions. This increases the QY to 0.93, which is now the highest for a monomeric fluorescent protein.
Goedhart et al. (2012)
Remarkably, introducing the super-folder GFP mutations (S30R, Y39N, N105T, and I171V) into mTurquoise2 yielded no apparent beneficial folding characteristics in either bacteria or mammalian cells, while the spectral properties were unchanged (Goedhart, unpublished observation).
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