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Comparison List

mCerulean

similar: Cerulean

mCerulean is a basic (constitutively fluorescent) cyan fluorescent protein published in 2005, derived from Aequorea victoria.
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Aggregation Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.8 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
433 475 43,000 0.64 27.52      

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
92.6 ± 2.0 (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

no photostability measurements available ... add one!

mCerulean Sequence

mCerulean was derived from Cerulean with the following mutations: A206K
amino acid numbers relative to avGFP. show relative to Cerulean

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

Excerpts

No excerpts have been added for mCerulean
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

High-Contrast Imaging of Fluorescent Protein FRET by Fluorescence Polarization Microscopy

Rizzo Ma, Piston Dw

(2005). Biophysical Journal, 88(2) , L14-L16. doi: 10.1529/biophysj.104.055442. Article   Pubmed

Additional References

  1. Two-photon absorption properties of fluorescent proteins

    Drobizhev M, Makarov Ns, Tillo Se, Hughes Te, Rebane A

    (2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article   Pubmed

External Resources

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