Comparison List

Superfolder GFP

a.k.a. sfGFP

Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer.
Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.8 kDa -

FPbase ID: B4SOW


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
488 510 83,300 0.65 54.15   13.6 3.1

Superfolder GFP OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
62.4 ± 6.3 (10000 cells) - HeLa Cranfill et al. (2016)
- 2.2 ± 0.1 (74 cells) U-2 OS Costantini et al. (2012)


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
208.26 80.0 (uW) Laser   idk Cranfill et al. (2016)
11.0 29.0 (W/cm2) Laser Other 25.0 Ivorra-Molla et al. (2023)

Superfolder GFP Sequence

Superfolder GFP was derived from Folding Reporter GFP with the following mutations: S30R/Y39N/N105T/Y145F/I171V/A206V

GenBank: ASL68970
IPG: 154375264


Deposited: ,


No excerpts have been added for Superfolder GFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. Site-Specific Fragmentation of Green Fluorescent Protein Induced by Blue Light

    Heckmeier Pj, Langosch D

    (2021). Biochemistry, 60(32) , 2457-2462. doi: 10.1021/acs.biochem.1c00248. Article   Pubmed

External Resources

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