| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Entacmaea quadricolor | 25.2 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 558 | 605 | 112,000 | 0.35 | 39.2 | 4.4 | 168.0 | 2.6 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 93.1 ± 2.1 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
No photostability measurements available ... add one!
mRuby was derived from eqFP611 with the following mutations: M12K/M15L/Y22H/D31E/V46I/K67R/H72Y/T73P/F102I/K120Q/T122R/L147M/S158T/Q159H/N163K/Y169H/E175V/E185G/F187I/F192I/F194A/K207N/H214R/C222A/D223G/P225_L231delinsGGG
In HEK293 cells expressing monomeric eqFP611 variants, the red fluorescence was not evenly distributed but rather appeared as dot-like structures in the cytoplasm... A similar effect was noticed for eqFP611 dimers that had the A/C interface disrupted. This result suggested that exposure of the A/C interface may have made a subcellular targeting signal accessible. Indeed, sequence analysis revealed that amino acids 229GRL231 at the C terminus, and also the preceding triplet 226SKL228 may serve as a type-1 peroxisomal targeting signal... We succeeded in removing peroxisomal targeting by replacing the C-terminal sequence 222CDLPSKLGRL231 of eqFP611 by 222AGLGGG227 [in mRuby]
Kredel et al. (2009)
Analysed by flow cytometry, the fluorescence signal of living HEK293 cells expressing the codon optimized variant [of mRuby] was 5–8 fold increased in comparison to the predecessor with unaltered codon usage but identical amino acid sequence.
Kredel et al. (2009)
(2009). PLoS ONE, 4(2) , e4391. doi: 10.1371/journal.pone.0004391. Article Pubmed
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