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Comparison List

mVenus

a.k.a. mVenus JBC

similar: Venus

mVenus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2006, derived from Aequorea victoria. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

FPbase ID: WCSN6

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
515 527 104,000 0.64 66.56 5.5 17.6 2.7

mVenus OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
83.9 ± 4.4 (10000 cells) - HeLa Cranfill et al. (2016)
83.9 ± 4.4 (10000 cells) - HeLa Hoi et al. (2013)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
10.1 62.5 (mW/mm2) LED Widefield 24.0 Lee et al. (2020)
50.4 20.0 (mW/mm2) LED Widefield HEK239A 24.0 Lee et al. (2020)
24.1 20.0 (mW/mm2) LED Widefield Saccharomyces cerevisiae 24.0 Lee et al. (2020)

mVenus Sequence

mVenus was derived from Venus with the following mutations: A206K
amino acid numbers relative to avGFP. show relative to Venus

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
GenBank: AAZ65844
IPG: 4596892

Excerpts

No excerpts have been added for mVenus
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. Versatile phenotype-activated cell sorting

    Lee J, Liu Z, Suzuki Ph, Ahrens Jf, Lai S, Lu X, Guan S, St-Pierre F

    (2020). Science Advances, 6(43) , eabb7438. doi: 10.1126/sciadv.abb7438. Article   Pubmed

External Resources

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