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Comparison List

Katushka

a.k.a. TurboFP635

Katushka is a basic (constitutively fluorescent) red fluorescent protein published in 2007, derived from Entacmaea quadricolor.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Entacmaea quadricolor 26.0 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
588 635 65,000 0.34 22.1      

Katushka OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
- (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
208.0 Widefield 25.0 Luker et al. (2015)

Katushka Sequence

Katushka was derived from TurboRFP with the following mutations: E3V/K6T/N21D/A68T/F110L/I115L/R138L/N143S/G152S/F174L/H193Y/H197R/K220R/R231S

Note: The sequence below matches the one shown in the primary reference (Shcherbo et al., 2007), but it commonly shown (e.g. at SnapGene) with an addition at the N-terminus: M1_S2insVGED

MSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTMKIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQMALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAVARYCDLPSKLGHS

Structure

Deposited: ,
Chromophore (MYG):

Excerpts

No excerpts have been added for Katushka
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

  1. Two-photon absorption properties of fluorescent proteins

    Drobizhev M, Makarov Ns, Tillo Se, Hughes Te, Rebane A

    (2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article   Pubmed

External Resources

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