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Comparison List

mCardinal

mCardinal is a basic (constitutively fluorescent) far red fluorescent protein published in 2014, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
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Aggregation Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 27.5 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
604 659 87,000 0.19 16.53 5.3 27.0  

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
41.3 ± 3.6 (10000 cells) - HeLa Cranfill et al. (2016)
41.8 ± 5.1 (150 cells) - - Bajar et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
730.0 Chu et al. (2014)

mCardinal Sequence

mCardinal was derived from mNeptune2 with the following mutations: S28T/G41Q/S143T
amino acid numbers relative to eqFP578. show relative to mNeptune2

MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQRIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK
GenBank: AHL19967
IPG: 49083567

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mCardinal
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

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External Resources

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