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Comparison List

TurboRFP

TurboRFP is a basic (constitutively fluorescent) orange fluorescent protein published in 2007, derived from Entacmaea quadricolor. It is reported to be a slowly-maturing dimer with low acid sensitivity.

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Aggregation Organism Molecular Weight Cofactor
Dimer Entacmaea quadricolor 26.1 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
553 574 92,000 0.67 61.64 4.4 90.0  

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
- (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
32.0 Strack et al. (2008)

TurboRFP Sequence

TurboRFP was derived from eqFP578 with the following mutations: R32G/T68A/L79F/L110F/S131P/L138R

MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVVEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTATQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKLVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCDLPSKLGHR

Excerpts

No excerpts have been added for TurboRFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

External Resources

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