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Comparison List

TurboRFP

TurboRFP is a basic (constitutively fluorescent) orange fluorescent protein published in 2007, derived from Entacmaea quadricolor. It has low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Entacmaea quadricolor 26.1 kDa -

FPbase ID: AHNXC

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
553 574 92,000 0.67 61.64 4.4 90.0  
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TurboRFP OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
- (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
32.0   Strack et al. (2008)
A caution on interpretation of photostability measurements
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TurboRFP Sequence

BLAST

TurboRFP was derived from eqFP578 with the following mutations: R32G/T68A/L79F/L110F/S131P/L138R

MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVVEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTATQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKLVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCDLPSKLGHR

Excerpts

No excerpts have been added for TurboRFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Merzlyak Em, Goedhart J, Shcherbo D, Bulina Me, Shcheglov As, Fradkov Af, Gaintzeva A, Lukyanov Ka, Lukyanov S, Gadella Twj, Chudakov Dm

(2007). Nature Methods, 4(7) , 555-557. doi: 10.1038/nmeth1062. Article   Pubmed

Additional References

  1. A noncytotoxic DsRed variant for whole-cell labeling

    Strack Rl, Strongin De, Bhattacharyya D, Tao W, Berman A, Broxmeyer He, Keenan Rj, Glick Bs

    (2008). Nature Methods, 5(11) , 955-957. doi: 10.1038/nmeth.1264. Article   Pubmed

External Resources

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