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Comparison List

mNeonGreen

a.k.a. mNG

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.
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Aggregation Organism Molecular Weight Cofactor
Monomer Branchiostoma lanceolatum 26.6 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
506 517 116,000 0.8 92.8 5.7 10.0 3.1

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
90.4 ± 2.1 (10000 cells) - HeLa Cranfill et al. (2016)
90.4 ± 2.1 (10000 cells) - HeLa Shaner et al. (2013)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
158.0 4.3 (mW) Arc-lamp Widefield H2B Shaner et al. (2013)

mNeonGreen Sequence

mNeonGreen was derived from dLanYFP with the following mutations: F15I/R25Q/Q56H/F67Y/K79V/S100V/F115A/V140R/T141S/M143K/L144T/D156K/T158S/Q168R/I185Y/F192Y
amino acid numbers relative to LanYFP. show relative to dLanYFP

MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK
GenBank: AGG56535
IPG: 34051755

Structure

Deposited: ,
Chromophore:

Excerpts

mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

Shaner et al. (2013)

... as it has so little sequence identity in common with other frequently used fluorescent proteins, mNeonGreen will be an attractive target for antibody development and should be amenable to orthogonal immunoprecipitation experiments along with jellyfish-derived and coral-derived fluorescent proteins.

Shaner et al. (2013)

Primary Reference

Additional References

External Resources

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