Comparison List


a.k.a. mNG

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Monomer Branchiostoma lanceolatum 26.6 kDa -



Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
506 517 116,000 0.8 92.8 5.7 10.0 3.1

mNeonGreen OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
90.4 ± 2.1 (10000 cells) - HeLa Shaner et al. (2013)
90.4 ± 2.1 (10000 cells) - HeLa Cranfill et al. (2016)


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
158.0 4.3 (mW) Arc-lamp Widefield H2B Shaner et al. (2013)
150.0 7.4 (W/cm2) Laser Spinning Disc Confocal HeLa (fixed) TPM2 25.0 Ivorra-Molla et al. (2023)

mNeonGreen Sequence

mNeonGreen was derived from dLanYFP with the following mutations: F15I/R25Q/Q56H/F67Y/K79V/S100V/F115A/V140R/T141S/M143K/L144T/D156K/T158S/Q168R/I185Y/F192Y
amino acid numbers relative to LanYFP. show relative to dLanYFP

GenBank: AGG56535
IPG: 34051755


Deposited: ,


mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

Shaner et al. (2013)

... as it has so little sequence identity in common with other frequently used fluorescent proteins, mNeonGreen will be an attractive target for antibody development and should be amenable to orthogonal immunoprecipitation experiments along with jellyfish-derived and coral-derived fluorescent proteins.

Shaner et al. (2013)

Notably, when both mTurquoise2 and mNeonGreen donors were included simultaneously, double-labeled cells could be found, which appeared to be capable of dividing (Figure 4—figure supplement 1), indicating that FP labeling does not affect cell viability in diploid CRISPIEd cells

Zhong et al. (2021)

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