|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|90.4 ± 2.1 (10000 cells)||-||HeLa||Shaner et al. (2013)|
|90.4 ± 2.1 (10000 cells)||-||HeLa||Cranfill et al. (2016)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|158.0||4.3 (mW)||Arc-lamp||Widefield||H2B||Shaner et al. (2013)|
mNeonGreen was derived from dLanYFP with the following mutations: F15I/R25Q/Q56H/F67Y/K79V/S100V/F115A/V140R/T141S/M143K/L144T/D156K/T158S/Q168R/I185Y/F192Y
amino acid numbers relative to LanYFP. show relative to dLanYFP
mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.
... as it has so little sequence identity in common with other frequently used fluorescent proteins, mNeonGreen will be an attractive target for antibody development and should be amenable to orthogonal immunoprecipitation experiments along with jellyfish-derived and coral-derived fluorescent proteins.
Notably, when both mTurquoise2 and mNeonGreen donors were included simultaneously, double-labeled cells could be found, which appeared to be capable of dividing (Figure 4—figure supplement 1), indicating that FP labeling does not affect cell viability in diploid CRISPIEd cells
(2021). eLife, 10, . doi: 10.7554/elife.64911. Article
(2017). , , . doi: 10.1101/160374. Article