David W Piston

1. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy

   Patterson Gh, Knobel Sm, Sharif Wd, Kain Sr, Piston Dw

   (1997). Biophysical Journal, 73(5) , 2782-2790. doi: 10.1016/s0006-3495(97)78307-3. Article

   Primary Proteins:

   Additional Proteins:

   1. αGFP
   2. avGFP
   3. EBFP
   4. EGFP
   5. GFP (S65T)

2. Improved Fluorescence and Dual Color Detection with Enhanced Blue and Green Variants of the Green Fluorescent Protein

   Yang T-T, Sinai P, Green G, Kitts Pa, Chen Y-T, Lybarger L, Chervenak R, Patterson Gh, Piston Dw, Kain Sr

   (1998). Journal of Biological Chemistry, 273(14) , 8212-8216. doi: 10.1074/jbc.273.14.8212. Article

   Primary Proteins:

   1. EBFP

   Additional Proteins:

   1. BFP5

3. An improved cyan fluorescent protein variant useful for FRET

   Rizzo Ma, Springer Gh, Granada B, Piston Dw

   (2004). Nature Biotechnology, 22(4) , 445-449. doi: 10.1038/nbt945. Article   Pubmed

   Primary Proteins:

   1. Cerulean
   2. D10
   3. ECFP H148D

   Additional Proteins:

   1. Citrine
   2. ECFP

4. High-Contrast Imaging of Fluorescent Protein FRET by Fluorescence Polarization Microscopy

   Rizzo Ma, Piston Dw

   (2005). Biophysical Journal, 88(2) , L14-L16. doi: 10.1529/biophysj.104.055442. Article   Pubmed

   Primary Proteins:

   1. mCerulean

5. Optimization of Pairings and Detection Conditions for Measurement of FRET between Cyan and Yellow Fluorescent Proteins

   Rizzo Ma, Springer G, Segawa K, Zipfel Wr, Piston Dw

   (2006). Microscopy and Microanalysis, 12(03) , 238-254. doi: 10.1017/s1431927606060235. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. mCerulean
   2. mCerulean3

6. X-ray Structure of Cerulean GFP:  A Tryptophan-Based Chromophore Useful for Fluorescence Lifetime Imaging†,‡

   Malo Gd, Pouwels Lj, Wang M, Weichsel A, Montfort Wr, Rizzo Ma, Piston Dw, Wachter Rm

   (2007). Biochemistry, 46(35) , 9865-9873. doi: 10.1021/bi602664c. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. Cerulean

7. Quantitative assessment of fluorescent proteins

   Cranfill Pj, Sell Br, Baird Ma, Allen Jr, Lavagnino Z, De Gruiter Hm, Kremers G-J, Davidson Mw, Ustione A, Piston Dw

   (2016). Nature Methods, 13(7) , 557-562. doi: 10.1038/nmeth.3891. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. mEGFP
   2. mEos4a
   3. mEos4b
   4. mTagBFP2