a.k.a. Midoriishi Cyan
similar: mMiCy
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Dimer | Acropora sp. #30 | 26.2 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 472 | 495 | 27,250 | 0.9 | 24.52 | 6.6 | 3.4 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 41.7 ± 10.7 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
No photostability measurements available ... add one!
Due to the high value and pH-resistance of the QE, MiCy would probably be a more efficient and reliable donor for FRET than ECFP. In addition, fluorescence lifetime measurement revealed that MiCy fluorescence displayed as a single exponential decay with a time constant of 3.4 ns.
KARASAWA et al. (2004)
Since all of the non-bioluminescent Anthozoan GFP-like proteins characterized so far form obligate tetramers (Labas et al 2002), the dimer formation of MiCy may indicate structural divergence of the protein family.
KARASAWA et al. (2004)
(2004). Biochemical Journal, 381(1) , 307-312. doi: 10.1042/bj20040321. Article Pubmed
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