a.k.a. Midoriishi Cyan
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|41.7 ± 10.7 (10000 cells)||-||HeLa||Cranfill et al. (2016)|
no photostability measurements available ... add one!
Due to the high value and pH-resistance of the QE, MiCy would probably be a more efficient and reliable donor for FRET than ECFP. In addition, fluorescence lifetime measurement revealed that MiCy fluorescence displayed as a single exponential decay with a time constant of 3.4 ns.
Since all of the non-bioluminescent Anthozoan GFP-like proteins characterized so far form obligate tetramers (Labas et al 2002), the dimer formation of MiCy may indicate structural divergence of the protein family.